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fibronectin collagen coating solution  (Thermo Fisher)


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    Thermo Fisher fibronectin collagen coating solution
    Fibronectin Collagen Coating Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibronectin+collagen+coating+solution/Collagen/pm41874140-240-24-34
    Average 99 stars, based on 1 article reviews
    fibronectin collagen coating solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Vape-Associated lncRNA Transcript 1 (VALT1) Amplifies the Tumorigenic Effects of e-Cigarette Vapor in Lung Epithelial Cells.
    Article Snippet: .. Immortalized BEAS-2B normal human bronchial epithelial cells (ATCC; Cat. No. CRL-3588) were maintained and cultured in T-75 culture flasks pre-coated with a 3 mL fibronectin-collagen coating solution consisting of 0.01 mg/mL human plasma fibronectin (Gibco®, Cat. No. 33016-015), 0.03 mg/mL bovine collagen I (Gibco®, Cat. No. A10644-01), and 0.01 mg/mL bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. A9418) in serum-free bronchial epithelial basal medium (BEBM; Lonza Bioscience, Walkersville, MD, USA, Cat. No. CC-3171) per ATCC guidelines. .. Serum-free LHC-9 medium (Gibco®, Cat. No. 12680-013) was used for the maintenance of BEAS-2B cells under controlled environmental conditions to prevent squamous differentiation (37 ◦C, 5% CO2).

    Article Title: Vape-Associated lncRNA Transcript 1 (VALT1) Amplifies the Tumorigenic Effects of e-Cigarette Vapor in Lung Epithelial Cells
    Article Snippet: .. Immortalized BEAS-2B normal human bronchial epithelial cells (ATCC; Cat. No. CRL-3588) were maintained and cultured in T-75 culture flasks pre-coated with a 3 mL fibronectin-collagen coating solution consisting of 0.01 mg/mL human plasma fibronectin (Gibco ® , Cat. No. 33016-015), 0.03 mg/mL bovine collagen I (Gibco ® , Cat. No. A10644-01), and 0.01 mg/mL bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. A9418) in serum-free bronchial epithelial basal medium (BEBM; Lonza Bioscience, Walkersville, MD, USA, Cat. No. CC-3171) per ATCC guidelines. .. Serum-free LHC-9 medium (Gibco ® , Cat. No. 12680-013) was used for the maintenance of BEAS-2B cells under controlled environmental conditions to prevent squamous differentiation (37 °C, 5% CO 2 ).

    Clinical Proteomics:

    Article Title: Vape-Associated lncRNA Transcript 1 (VALT1) Amplifies the Tumorigenic Effects of e-Cigarette Vapor in Lung Epithelial Cells.
    Article Snippet: .. Immortalized BEAS-2B normal human bronchial epithelial cells (ATCC; Cat. No. CRL-3588) were maintained and cultured in T-75 culture flasks pre-coated with a 3 mL fibronectin-collagen coating solution consisting of 0.01 mg/mL human plasma fibronectin (Gibco®, Cat. No. 33016-015), 0.03 mg/mL bovine collagen I (Gibco®, Cat. No. A10644-01), and 0.01 mg/mL bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. A9418) in serum-free bronchial epithelial basal medium (BEBM; Lonza Bioscience, Walkersville, MD, USA, Cat. No. CC-3171) per ATCC guidelines. .. Serum-free LHC-9 medium (Gibco®, Cat. No. 12680-013) was used for the maintenance of BEAS-2B cells under controlled environmental conditions to prevent squamous differentiation (37 ◦C, 5% CO2).

    Article Title: Vape-Associated lncRNA Transcript 1 (VALT1) Amplifies the Tumorigenic Effects of e-Cigarette Vapor in Lung Epithelial Cells
    Article Snippet: .. Immortalized BEAS-2B normal human bronchial epithelial cells (ATCC; Cat. No. CRL-3588) were maintained and cultured in T-75 culture flasks pre-coated with a 3 mL fibronectin-collagen coating solution consisting of 0.01 mg/mL human plasma fibronectin (Gibco ® , Cat. No. 33016-015), 0.03 mg/mL bovine collagen I (Gibco ® , Cat. No. A10644-01), and 0.01 mg/mL bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. A9418) in serum-free bronchial epithelial basal medium (BEBM; Lonza Bioscience, Walkersville, MD, USA, Cat. No. CC-3171) per ATCC guidelines. .. Serum-free LHC-9 medium (Gibco ® , Cat. No. 12680-013) was used for the maintenance of BEAS-2B cells under controlled environmental conditions to prevent squamous differentiation (37 °C, 5% CO 2 ).



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    Image Search Results


    Cell affinity between TMSCs-TM cells and between TMSCs-matrix. (A) Fibronectin (FN) or a combination of DiO-labeled TM cells (green) and fibronectin (FN+TM) served as substrates. DiI-labeled red TMSCs, TMSCs treated with anti-α5β1 integrin antibody (TMSC+ α5β1 Integrin↓) or TMSCs treated with IgG (TMSC+IgG) were seeded on top of FN, TM+FN, or directly onto culture plates as None for 1 h. DAPI stains nuclei blue. Scale bars 50 μm. (B) Attached TMSC numbers per field averaged from three individual experiments with at least five fields per condition. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way ANOVA followed by Bonferroni's multiple comparisons test. TM, trabecular meshwork. Color images are available online.

    Journal: Stem Cells and Development

    Article Title: α5β1 Integrin Promotes Anchoring and Integration of Transplanted Stem Cells to the Trabecular Meshwork in the Eye for Regeneration

    doi: 10.1089/scd.2019.0254

    Figure Lengend Snippet: Cell affinity between TMSCs-TM cells and between TMSCs-matrix. (A) Fibronectin (FN) or a combination of DiO-labeled TM cells (green) and fibronectin (FN+TM) served as substrates. DiI-labeled red TMSCs, TMSCs treated with anti-α5β1 integrin antibody (TMSC+ α5β1 Integrin↓) or TMSCs treated with IgG (TMSC+IgG) were seeded on top of FN, TM+FN, or directly onto culture plates as None for 1 h. DAPI stains nuclei blue. Scale bars 50 μm. (B) Attached TMSC numbers per field averaged from three individual experiments with at least five fields per condition. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way ANOVA followed by Bonferroni's multiple comparisons test. TM, trabecular meshwork. Color images are available online.

    Article Snippet: Fibronectin (FN) coating group: 0.5 mL of fibronectin and collagen (FNC)-coating solution (Athena ES, Baltimore, MD) containing 10 μg/mL FN was coated at room temperature for 3 h on 8-chamber slides.

    Techniques: Labeling

    Expression of fibronectin in murine TM. (A) Immunostaining of fibronectin (red) in the TM of normal mice. The positive signal for endothelial cells of Schlemm's canal and vessels displaying green (CD31 staining). (B) Fibronectin staining (red) in the TM of laser-treated mice at 2 weeks. DAPI stains nuclei blue. Scale bars 50 μm. (C) Quantification of mean fluorescent intensity of fibronectin expression in normal and laser-treated mouse TM in three to five samples. *P < 0.05. Unpaired t-test. CO, cornea; SC, Schlemm's canal. Color images are available online.

    Journal: Stem Cells and Development

    Article Title: α5β1 Integrin Promotes Anchoring and Integration of Transplanted Stem Cells to the Trabecular Meshwork in the Eye for Regeneration

    doi: 10.1089/scd.2019.0254

    Figure Lengend Snippet: Expression of fibronectin in murine TM. (A) Immunostaining of fibronectin (red) in the TM of normal mice. The positive signal for endothelial cells of Schlemm's canal and vessels displaying green (CD31 staining). (B) Fibronectin staining (red) in the TM of laser-treated mice at 2 weeks. DAPI stains nuclei blue. Scale bars 50 μm. (C) Quantification of mean fluorescent intensity of fibronectin expression in normal and laser-treated mouse TM in three to five samples. *P < 0.05. Unpaired t-test. CO, cornea; SC, Schlemm's canal. Color images are available online.

    Article Snippet: Fibronectin (FN) coating group: 0.5 mL of fibronectin and collagen (FNC)-coating solution (Athena ES, Baltimore, MD) containing 10 μg/mL FN was coated at room temperature for 3 h on 8-chamber slides.

    Techniques: Expressing, Immunostaining, Staining